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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/07/2023 |
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Actualizado : |
24/07/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
GIANNEECHINI, R.; CONCHA, C.; DELUCCHI, M.I.; GIL, J.; SALVARREY, L.; RIVERO, R. |
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Afiliación : |
R. GIANNEECHINI, Laboratorio Regional Noroeste DILAVE "Miguel C. Rubino", MGAP CC 57037, CP 60000, Paysandú, Uruguay; Departmento de Ciencias Microbiológicas, Área de Bacteriología, Facultad de Veterinaria, Univ. de la República, Regional Norte, Salto; C. CONCHA, Departamento de Producción Animal, Facultad de Ciencias Agrícolas, Universidad de Chile, Santiago, Chile.; MARIA INES DELUCCHI ZAPARRART, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; J. GIL, Departmento de Salud en los Sistemas Pecuarios, Facultad de Veterinaria, Universidad de la República, Paysandú, Uruguay.; L. SALVARREY, Departmento de Bioestadística, Facultad de Veterinaria, Universidad de la República, Regional Norte, Salto, Uruguay.; R. RIVERO, Laboratorio Regional Noroeste DILAVE “Miguel C. Rubino”, MGAP CC 57037, CP 60000, Paysandú, Uruguay. |
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Título : |
Mastitis bovina, reconocimiento de los patógenos y su resistencia antimicrobiana en la Cuenca Lechera del Sur de Uruguay. [Bovine mastitis, distribution of pathogens and antimicrobial resistance in the Southern Dairy Basin of Uruguay.] |
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Complemento del título : |
Sección: Artículos originales. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Veterinaria (Montevideo), 2014, v. 50, no. 196, p. 4-32. -- OPEN ACCESS. |
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ISSN : |
0376-4362 (impresa); 1688-4809 (en línea). |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido 27 Setiembre 2013; Aprobado 14 Enero 2014; Publicado 1 Diciembre 2014. -- Autor de correspondencia: mailto:egianneechini@mgap.gub.uy -- -- El trabajo fue realizado dentro del proyecto FPTA 94 con fondos otorgados por el Instituto Nacional de Investigaciones Agropecuarias (INIA), Uruguay. -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU). |
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Contenido : |
RESUMEN.- Cincuenta y tres establecimientos lecheros del sur de Uruguay fueron seleccionados para determinar la prevalencia de mastitis subclínicas, la incidencia de mastitis clínica, distribución de los patógenos y su susceptibilidad antimicrobiana. La prevalencia de mastitis subclínica fue determinada en base al recuento de células somáticas en 9.016 muestras de leche obtenidas de 2.254 vacas de acuerdo a un umbral >300.000 células/ mL. La prevalencia media fue de 54,2% animales afectados, siendo Staphylococcus aureus (21,4%) el principal patógeno aislado de muestras positivas. Durante un año fueron registrados 667 casos clínicos, determinándose la incidencia media de mastitis clínica en 11,8 casos cada 100 vacas/año en riesgo. Un total de 341 muestras de leche fueron obtenidas de los casos registrados y el patógeno más frecuentemente aislado fue Staphylococcus aureus (27,8%). Un total de 864 aislamientos de los géneros estafilococo, estreptococo y enterococo obtenidos de casos de mastitis clínica y subclínica fueron analizados en su susceptibilidad a agentes antimicrobianos por el método de Disco Difusión Agar. Un 39,1% de aislamientos de Staphylococcus aureus obtenidos de casos subclínicos y un 36% de casos clínicos fueron resistentes a la penicilina, y en Staphylococcus coagulasa negativos el 29,4% y 33,3%, respectivamente. Los aislamientos del género estreptococo resultaron susceptibles a penicilina, mientras que el 12,5% del género enterococo fueron resistentes. En conclusión, Uruguay mantiene un alto nivel de prevalencia de casos de mastitis subclínica en el rodeo lechero. Staphylococcus aureus es confirmado como el principal agente etiológico con un alto porcentaje de resistencia a penicilina. ------------- SUMMARY.- A total of 53 farms were selected in Southern dairy region of Uruguay, in order to estimate the prevalence of subclinical mastitis, the incidence of clinical mastitis, distribution and antimicrobial susceptibility of mastitis pathogens. The prevalence of sub-clinical mastitis was determined in base on somatic cell counts in quarter?s milk samples and a threshold value of > 300,000 cells/ mL.; 9,016 milk quarters samples were collected from 2,254 dairy cows and the mean prevalence at herd level was 54.2% of affected cows. The main pathogen isolated from positive samples was Staphylococcus aureus (21.4%). During one year 667 cases of clinical mastitis were recorded with a mean incidence rate of clinical mastitis of 11.8 cases per 100 cow/year at risk. Milk samples were obtained from 341 clinical cases for bacteriological isolation, the most prevalent pathogen isolated was Staphylococcus aureus (27.8%). A total of 864 strains belonging to staphylococci, streptococci and enterococci isolated from clinical and subclinical mastitis cases were analyzed for their susceptibility to antimicrobial agents. The susceptibility patterns were studied by Agar disk diffusion method. The 39.1% of Staphylococcus aureus strains from subclinical cases and 36% from clinical cases were resistant to penicillin, and 29.4% and 33.3% of coagulase negative staphylococci strains, respectively. All the streptococcal isolates were susceptible to penicillin, while 12.5% of enterococci strains were resistant. In conclusion, Uruguay maintain a high level of sub clinical infection in the dairy herds, and Staphylococcus aureus is confirmed as the principal pathogen with a high percentage of penicillin resistance. MenosRESUMEN.- Cincuenta y tres establecimientos lecheros del sur de Uruguay fueron seleccionados para determinar la prevalencia de mastitis subclínicas, la incidencia de mastitis clínica, distribución de los patógenos y su susceptibilidad antimicrobiana. La prevalencia de mastitis subclínica fue determinada en base al recuento de células somáticas en 9.016 muestras de leche obtenidas de 2.254 vacas de acuerdo a un umbral >300.000 células/ mL. La prevalencia media fue de 54,2% animales afectados, siendo Staphylococcus aureus (21,4%) el principal patógeno aislado de muestras positivas. Durante un año fueron registrados 667 casos clínicos, determinándose la incidencia media de mastitis clínica en 11,8 casos cada 100 vacas/año en riesgo. Un total de 341 muestras de leche fueron obtenidas de los casos registrados y el patógeno más frecuentemente aislado fue Staphylococcus aureus (27,8%). Un total de 864 aislamientos de los géneros estafilococo, estreptococo y enterococo obtenidos de casos de mastitis clínica y subclínica fueron analizados en su susceptibilidad a agentes antimicrobianos por el método de Disco Difusión Agar. Un 39,1% de aislamientos de Staphylococcus aureus obtenidos de casos subclínicos y un 36% de casos clínicos fueron resistentes a la penicilina, y en Staphylococcus coagulasa negativos el 29,4% y 33,3%, respectivamente. Los aislamientos del género estreptococo resultaron susceptibles a penicilina, mientras que el 12,5% del género enterococo fueron resistentes. En co... Presentar Todo |
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Palabras claves : |
Dairy cow; Incidence; Incidencia; Prevalence; Prevalencia. |
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Thesagro : |
MASTITIS; VACAS LECHERAS. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17300/1/Gianneechini-et-al.-2014.-SMVU-v50-n196.pdf
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Marc : |
LEADER 04845naa a2200289 a 4500
001 1064265
005 2023-07-24
008 2014 bl uuuu u00u1 u #d
022 $a0376-4362 (impresa); 1688-4809 (en línea).
100 1 $aGIANNEECHINI, R.
245 $aMastitis bovina, reconocimiento de los patógenos y su resistencia antimicrobiana en la Cuenca Lechera del Sur de Uruguay. [Bovine mastitis, distribution of pathogens and antimicrobial resistance in the Southern Dairy Basin of Uruguay.]$h[electronic resource]
260 $c2014
500 $aArticle history: Recibido 27 Setiembre 2013; Aprobado 14 Enero 2014; Publicado 1 Diciembre 2014. -- Autor de correspondencia: mailto:egianneechini@mgap.gub.uy -- -- El trabajo fue realizado dentro del proyecto FPTA 94 con fondos otorgados por el Instituto Nacional de Investigaciones Agropecuarias (INIA), Uruguay. -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).
520 $aRESUMEN.- Cincuenta y tres establecimientos lecheros del sur de Uruguay fueron seleccionados para determinar la prevalencia de mastitis subclínicas, la incidencia de mastitis clínica, distribución de los patógenos y su susceptibilidad antimicrobiana. La prevalencia de mastitis subclínica fue determinada en base al recuento de células somáticas en 9.016 muestras de leche obtenidas de 2.254 vacas de acuerdo a un umbral >300.000 células/ mL. La prevalencia media fue de 54,2% animales afectados, siendo Staphylococcus aureus (21,4%) el principal patógeno aislado de muestras positivas. Durante un año fueron registrados 667 casos clínicos, determinándose la incidencia media de mastitis clínica en 11,8 casos cada 100 vacas/año en riesgo. Un total de 341 muestras de leche fueron obtenidas de los casos registrados y el patógeno más frecuentemente aislado fue Staphylococcus aureus (27,8%). Un total de 864 aislamientos de los géneros estafilococo, estreptococo y enterococo obtenidos de casos de mastitis clínica y subclínica fueron analizados en su susceptibilidad a agentes antimicrobianos por el método de Disco Difusión Agar. Un 39,1% de aislamientos de Staphylococcus aureus obtenidos de casos subclínicos y un 36% de casos clínicos fueron resistentes a la penicilina, y en Staphylococcus coagulasa negativos el 29,4% y 33,3%, respectivamente. Los aislamientos del género estreptococo resultaron susceptibles a penicilina, mientras que el 12,5% del género enterococo fueron resistentes. En conclusión, Uruguay mantiene un alto nivel de prevalencia de casos de mastitis subclínica en el rodeo lechero. Staphylococcus aureus es confirmado como el principal agente etiológico con un alto porcentaje de resistencia a penicilina. ------------- SUMMARY.- A total of 53 farms were selected in Southern dairy region of Uruguay, in order to estimate the prevalence of subclinical mastitis, the incidence of clinical mastitis, distribution and antimicrobial susceptibility of mastitis pathogens. The prevalence of sub-clinical mastitis was determined in base on somatic cell counts in quarter?s milk samples and a threshold value of > 300,000 cells/ mL.; 9,016 milk quarters samples were collected from 2,254 dairy cows and the mean prevalence at herd level was 54.2% of affected cows. The main pathogen isolated from positive samples was Staphylococcus aureus (21.4%). During one year 667 cases of clinical mastitis were recorded with a mean incidence rate of clinical mastitis of 11.8 cases per 100 cow/year at risk. Milk samples were obtained from 341 clinical cases for bacteriological isolation, the most prevalent pathogen isolated was Staphylococcus aureus (27.8%). A total of 864 strains belonging to staphylococci, streptococci and enterococci isolated from clinical and subclinical mastitis cases were analyzed for their susceptibility to antimicrobial agents. The susceptibility patterns were studied by Agar disk diffusion method. The 39.1% of Staphylococcus aureus strains from subclinical cases and 36% from clinical cases were resistant to penicillin, and 29.4% and 33.3% of coagulase negative staphylococci strains, respectively. All the streptococcal isolates were susceptible to penicillin, while 12.5% of enterococci strains were resistant. In conclusion, Uruguay maintain a high level of sub clinical infection in the dairy herds, and Staphylococcus aureus is confirmed as the principal pathogen with a high percentage of penicillin resistance.
650 $aMASTITIS
650 $aVACAS LECHERAS
653 $aDairy cow
653 $aIncidence
653 $aIncidencia
653 $aPrevalence
653 $aPrevalencia
700 1 $aCONCHA, C.
700 1 $aDELUCCHI, M.I.
700 1 $aGIL, J.
700 1 $aSALVARREY, L.
700 1 $aRIVERO, R.
773 $tVeterinaria (Montevideo), 2014$gv. 50, no. 196, p. 4-32. -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
31/07/2018 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DOSTER, E.; ROVIRA, P.J.; NOYES, N.R.; BURGESS, B. A.; YANG, X.; WEINROTH, M.D.; LAKIN, S.M.; DEAN, C.J.; LINKE, L.; MAGNUSON, R.; JONES, K.I.; BOUCHER, C.; RUIZ, J.; BELK, K.E.; MORLEY, P.S. |
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Afiliación : |
ENRIQUE DOSTER, Microbial Ecology Group, Colorado State University, USA. Department of Microbiology, Inmunology and Pathology, Colorado State University. USA.; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Microbial Ecology Group, Colorado State University. Department of Microbiology, Imnunology and Pathology, Colorado State University, USA.; NOELLE R. NOYES., Microbial Ecology Group, Colorado State University. Department of Veterinary Population Medicine, University of Minnesota.; BRANDY A. BURGESS, Department of Population Health, University of Georgia, USA.; XIANG YANG, Microbial Ecology Group, Colorado State University. Department of Animal Sciences, Colorado State University, USA.; MARGARET D. WEINROTH, Microbial Ecology Group, Colorado State University. Department of Animal Sciences, Colorado State University, USA.; STEVEN M. LAKIN, Microbial Ecology Group, Colorado State University. Department of Microbiology, Immunology and Pathology, Colorado State University, USA.; CHRISTOPHER J. DEAN, Microbial Ecology Group, Colorado State University. Department of Microbiology, Immunology and Pathology, Colorado State University, USA.; LYNDSEY LINKE, Department of Clinical Sciences, Colorado State University, USA.; ROBERTA MAGNUSON, Department of Clinical Sciences, Colorado State University, USA.; KENNETH I. JONES, Department of Biochemistry and Molecular Genetics, University of Colorado, USA.; CHRISTINA BOUCHER, Department of Computer and Information Science and Engineering, University of Florida, USA.; JAMIE RUIZ, Department of Computer and Information Science and Engineering, University of Florida, USA.; KEITH E. BELK, Microbial Ecology Group, Colorado State University. Department of Microbiology, Imnunology and Pathology, Colorado State University, USA.; PAUL S. MORLEY, Microbial Ecology Group, Colorado State University, USA. Department of Microbiology, Inmunology and Pathology, Colorado State University. USA. |
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Título : |
Investigating effects of tulathromycin metaphylaxis on the fecal resistome and microbiome of commercial feedlot cattle early in the feeding period. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontier in Microbiology, 2018, 9:1715. |
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Páginas : |
14 p. |
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DOI : |
10.3389/fmicb.2018.01715 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 14 April 2018; Accepted: 09 July 2018; Published: 30 July 2018.
Open Access journal.
https://doi.org/10.3389/fmicb.2018.01715 |
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Contenido : |
The objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography, conspecific exposure, and environment?may exert a greater influence over the fecal resistome and microbiome of feedlot cattle than common metaphylactic antimicrobial drug treatment. MenosThe objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography... Presentar Todo |
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Palabras claves : |
METAGENOMICS; METAPHYLAXIS; MICROBIOME; RESISTOME; TULATHROMYCIN. |
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Thesagro : |
BOVINOS; FEEDLOT. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10933/1/fmicb-09-01715.pdf
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Marc : |
LEADER 02931naa a2200409 a 4500
001 1058855
005 2019-10-11
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2018.01715$2DOI
100 1 $aDOSTER, E.
245 $aInvestigating effects of tulathromycin metaphylaxis on the fecal resistome and microbiome of commercial feedlot cattle early in the feeding period.$h[electronic resource]
260 $c2018
300 $a14 p.
500 $aArticle history: Received: 14 April 2018; Accepted: 09 July 2018; Published: 30 July 2018. Open Access journal. https://doi.org/10.3389/fmicb.2018.01715
520 $aThe objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography, conspecific exposure, and environment?may exert a greater influence over the fecal resistome and microbiome of feedlot cattle than common metaphylactic antimicrobial drug treatment.
650 $aBOVINOS
650 $aFEEDLOT
653 $aMETAGENOMICS
653 $aMETAPHYLAXIS
653 $aMICROBIOME
653 $aRESISTOME
653 $aTULATHROMYCIN
700 1 $aROVIRA, P.J.
700 1 $aNOYES, N.R.
700 1 $aBURGESS, B. A.
700 1 $aYANG, X.
700 1 $aWEINROTH, M.D.
700 1 $aLAKIN, S.M.
700 1 $aDEAN, C.J.
700 1 $aLINKE, L.
700 1 $aMAGNUSON, R.
700 1 $aJONES, K.I.
700 1 $aBOUCHER, C.
700 1 $aRUIZ, J.
700 1 $aBELK, K.E.
700 1 $aMORLEY, P.S.
773 $tFrontier in Microbiology, 2018, 9:1715.
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